The absence of Melbourne product Sione Tuipulotu is one of two of the changes to the British and Irish Lions squad to face the Wallabies in the second Test.

Tuipulotu, who scored a try after he started in the centres in game one in Brisbane, was robbed of a fairytale homecoming when he left out of match-day 23 as the Lions try to wrap up the series at the MCG on Saturday.

A Melbourne schoolboy before shifting to Scotland where he captains the national side, Tuipulotu was ruled out with a tight hamstring with Ireland's Bundee Aki named at 12.

Coach Andy Farrell said the 28-year-old, who qualified for Scotland through his grandmother, was shattered to miss out on playing in front of 90,000 in Melbourne.

"When you've got a little bit of a tight hamstring, it influences how you're possibly going to go, so we made the call to go with Bundee," Farrell said.

"100 per cent ( Tuipulotu) is absolutely gutted. He also knows that that's rugby, fairytales are not always written.

"Sione is a team man anyway. And it's the same with anyone who's missed out. Every single one of them has been rowing forward by putting the team first."

Coach Farrell said they were expecting a "massive reaction" from the Wallabies after their Suncorp Stadium disappointment.

"We have put ourselves in a good position after the first Test, but we know there will be a massive reaction from this Wallaby team," Farrell said.

"Everyone saw the quality they have in Brisbane and we know we will have to be a lot better than we were last week."

Melissa Woods - AAP