Opposition leader Peter Dutton has expelled Senator David Van from the federal Liberal party room after allegations of sexual assault were made against him by Senator Lidia Thorpe in the Senate on Wednesday evening.

On Thursday, Mr Dutton said further allegations had been made against Senator Van and he had removed him from the party room without "making any judgement on… any individual's guilt or innocence".

"Since the airing of Senator Thorpe's allegation yesterday, further allegations in relation to Senator Van have been brought to my attention overnight and this morning. As such, I met with Senator Van this morning and a short time ago, I advised Senator Van of my decision that he should no longer sit in the Liberal party party room," Mr Dutton said in a statement.

"At the outset, I want to make clear, very clear, that I'm not making any judgement on the veracity of the allegations or any individual's guilt or innocence."

The decision came on the same day Senator Thorpe detailed allegations of incidents in Parliament House by unnamed perpetrators, and expressed her disappointment at Senator Van not "stepping up and taking accountability" for the allegations.

On Wednesday evening Ms Thorpe withdrew her comments against Mr Van in the chamber, understood to be a matter of protocol, but did not withdraw her allegations more broadly.

Senator Van has repeatedly denied the claims against him.

Through tears, Senator Thorpe told the Senate that Parliament House is "not a safe space", in detailing her alleged experiences of "sexual comments" and being "inappropriately propositioned by powerful men".

"Yesterday I made remarks in relation to another senator. I then had to withdraw them because the rules of the Senate do not allow you to speak about someone's character, only about something they have said. So today, I will speak about my experience in Parliament," Ms Thorpe said.

"When I started, I was a new senator, as all women that have walked the corridors of this building know, it is not a safe place.

"You are often alone in long corridors with no windows and in stairwells hidden from view where there are no cameras. This was my new workplace. This is the workplace women in this building know.

"I experienced sexual comments and was inappropriately propositioned by powerful men. One man followed me and cornered me in a stairwell."

Senator Thorpe said staff and fellow members witnessed "most of this" but that there were no cameras in stairwells.

She added that other women have endured similar experiences but not come forward in order to protect their careers.

"There are different understandings of what amounts to sexual assault," Senator Thorpe said.

"What I experienced was being followed aggressively, propositioned and inappropriately touched or was afraid to walk out of the office door. I would open the door slightly and check the place was clear before stepping out…to me, it was sexual assault."

The Gunditjmara, Gunnai and Djab Wurrung senator thanked her former Greens colleagues for escorting her through the building as a result of the alleged incidents.

Senator Thorpe said she did not speak publicly at the time of the alleged incidents so as not to take away from Brittany Higgins rape allegations, but said she could not longer sit in silence.

"Silence is violence, and yesterday I could not stay silent. As someone who has knowingly made me feel unsafe, had the gall to stand up in front of Parliament and preach about protecting women. This was not an isolated incident. And there are others I could name who have inappropriately touched me, invaded my space and knowingly made me feel unsafe," she said.

The independent senator for Victoria then expressed her "disappointment" that Senator Van had not taken accountability for the allegations made against him.

"I'm disappointed by the reaction of the senator instead of stepping up (and) taking accountability for the fact that he made me feel unsafe. He denied it…this type of behaviour makes it harder for other women to come forward."

On Thursday, Senator Van acknowledged he had previously moved offices in 2021 after earlier allegations from Senator Thorpe.

He stated he denied the allegations at the time and continues to do so.

"I agreed to move offices to ensure the avoidance of any misconception. No incident was ever alleged by her. And I agreed to protect myself against her irrational concerns and ensure the effective and smooth running of the parliament," he said in both a statement and in the Senate on Wednesday.

He later accepted Mr Dutton's move to remove him from the party room.

"I do not wish this matter to stay in the Liberal party that I have fought so hard for, so I accept that I will no longer be sitting in the party room."

Senator Van called for an investigation into the "outrageous claims" against him, and disputed an earlier claim in Senator Thorpe's speech that his lawyer had acted in writing at the time.

"There should be and must be an investigation into these outrageous claims so that they can be proved to be false. I will fully cooperate with the investigators and answer any questions that they may have of me and Senator Thorpe should do the same."

"Finally, the allegation of intimidation by my lawyer is unfounded."